Abstract
An enzyme-free immunosorbent assay using MgFe2O4 modified with cysteine (MgFe2O4@Cys) as sacrificial probe for the detection of procalcitonin (PCT) was developed. MgFe2O4@Cys was linked with second antibody of PCT (Ab2) by cysteine amino group and released Fe3+ under acidic conditions. Taking advantage of Fe3+ and [Fe(CN)6]4− generating soluble Prussian blue (PB), a novel method of PCT determination was established by measuring the absorbance of the generated PB. Different from the traditional immunosensor detection mode, the enzyme-free colorimetric immunosensor in this work can not only avoid the interference of nano-enzymes or enzymes, but also has the advantages of rapid detection, low cost, high selectivity and sensitivity. Therefore, the developed immunosensor for PCT detection exhibited a wide linear response (0.0001–50 ng/mL) and a low detection limit (44.9 fg/mL). Additionally, the immunosensor exhibits satisfactory performance in human serum testing, which indicated its good application value in clinical diagnoses.
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