Abstract
Nearest neighbor analysis of immunocytolocalization experiments indicates that the Embden–Meyerhof pathway enzymes aldolase, triose-P isomerase, glyceraldehyde-3-P dehydrogenase and P-glycerate kinase are located close to one another in the pea leaf cytosol. Direct transfer of the triose phosphates between aldolase, glyceraldehyde-3-P dehydrogenase and triose-P isomerase, direct transfer of glyceraldehyde-3-P between glyceraldehyde-3-P dehydrogenase and triose-P isomerase, and direct transfer of 1,3-P 2-glycerate between glyceraldehyde-3-P dehydrogenase and P-glycerate kinase is then a possibility in the plant cytosol. In contrast, there is no indication that fructose bisphosphatase is co-localized with aldolase, and it unlikely that fructose bisphosphate is channeled from aldolase to fructose bisphosphatase in this system.
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