Abstract
Carboxylic ester hydrolases in human serum were identified, characterized, and quantified by means of crossed and rocket immunoelectrophoresis. Two types of esterase were found, arylesterase (EC 3.1.1.2) and cholinesterase (EC 3.1.1.8), exhibiting differences in specificity for several histochemical substrates. α‐naphthyl acetate and β‐naphthyl acetate were used to distinguish between the two enzymes. Arylesterase seemed to interact specifically with aJipoprotein. Quantitation of enzyme protein and correlation with enzyme activity is described. The experiments illustrate general methodological problems concerning immunochemical characterization of enzymes.
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