Enzyme-based sensing on nanohybrid film coated over FTO electrode for highly sensitive detection of antibiotics.

Abstract

Cefepime and Meropenem are the new class of antibiotics, which are particularly used as last potent defender or the antibiotics of the last resort against multi-resistant bacterial species. In this paper, an impedance-based electrochemical biosensor was fabricated for identifying antibiotics of last resort in the forensic samples including gastric lavage and other body fluids. The sensor was developed using platinum nanoparticles (PtNPs) and electrodeposited zinc oxide- zinc hexacyanoferrate hybrid film (ZnO/ZnHCF) on the surface of a fluorine-doped glass electrode (FTO). Further, penicillinase was immobilized onto the modified electrode using penicillinase enzyme. The developed biosensor exhibits agood analytical response for the detection of antibiotics evaluated using electrochemistry studies. The linear response of thefabricated electrode was observed from 0.1 to 750µM and the electrode limit of detection (LOD) was observed as 0.1µM. The sensor confirms good accuracy, ishighly selective, and sensitive for the target. While storingthe modified electrode at 4°C, the stability of biosensor was evaluated for 45days, and activityloss of 30-40% was observed. Thehighly sensitive interface of Penicillinase@CHIT/PtNP-ZnO/ZnHCF/FTO electrode shows a promising future in forensic studies.