Abstract
Enzyme-assisted extraction (EAE) has been successfully applied to extract polyphenols from various food materials and by-products. Hence, the present research aimed to valorize the cashew nut (Anacardium occidentale L.) testa by EAE method using an enzyme cocktail of cellulase and pectinase. The effects of different processing parameters, including the pH (3.0 - 6.0), temperature (30 - 70°C), extraction time (60 - 150 min), solid -to-solvent ratio (1/5 - 1/85), and enzyme concentration (0.1 - 0.4% v/v) on the total polyphenol content (TPC) and antioxidant capacity of the testa extracts were investigated. The results showed that the TPC of the testa extracts positively correlated with the antioxidant capacity. The EAE performed at pH 4.0, a temperature of 50°C, an extraction time of 60 mins, a solid-to-solvent ratio of 1/55, and an enzyme concentration of 0.2% (v/ v) resulted in the highest TPC (168.84 g GAE/kg DW) with DPPH and ABTS antioxidant activity of 885.56 and 1513.60 (mmol TE/kg DW), respectively. The EAE technique holds significant promise as a sustainable approach to mitigate the underutilization and undervaluation of cashew nut testa waste, which could otherwise contribute to environmental pollution.
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