Enzyme and protein mass transfer coefficient in aqueous two-phase systems—I. Spray extraction columns

Abstract

Fractional dispersed phase hold-up and dispersed side mass transfer coefficients for bovine serum albumin (BSA) and amyloglucosidase were measured in 22, 34, 50, 56, 70 and 95 mm i.d. spray columns using salt-polyethylene glycol (potassium phosphate-PEG and sodium sulphate-PEG) systems. The effect of distributor design and column height were investigated. The effect of phase compositions of the aqueous phase system was also studied. Empirical and semi-empirical correlations have been developed for fractional dispersed phase hold-up and dispersed side mass transfer coefficients.