Abstract
ConclusionsWhile some work remains to be done to establish that these new TK variants function firmly as super suicide genes in vivo, it is important to recognize that they contain multiple amino acid substitutions and would likely never be made by site-directed mutagenesis. Clearly, these results demonstrate the power of random sequence mutagenesis to tailor enzymes such as cytosine deaminase and HSV-1 thymidine kinase for gene therapy of cancers. Mutant HSV-1 TKs also hold promise for use in a wide variety of other applications including as a negative selectable marker for homologous recombination events for cell lineage ablation investigations and for tumor imaging by positron emission tomography. Random sequence mutagenesis coupled with positive genetic selection is a powerful means not only to investigate the contributions of a particular residue to function but also for the generation of enzymes with novel functions for numerous biomedical applications.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
