Enzyme and metabolites attained in degradation of chemical pesticides ß Cypermethrin by Bacillus cereus

Abstract

The aim of this investigation was to find enzymes and metabolites involved in ß Cypermethrin pesticide degradation. The continuous usage of chemical pesticide for rapid agriculture activities leads to the major cause of soil pollution. In this study, Bacillus cereus, a bacterium isolated from the BT cotton cultivated and pesticide contaminated soil has the efficiency to degrade ß Cypermethrin, a synthetic pyrethroid insecticide used in most crop cultivations. Standard lab scale bioremediation process was followed on mineral salt agar medium (MSM) enriched with ß Cypermethrin. Initially, the fragmentation of ß Cypermethrin was confirmed by Thin-Layer Chromatography (TLC), which revealed the existence of metabolites with Retention Factor (RF) value range of 0.47 and 0.71, corresponding to standard samples of 3-PBA and phenol. Later, SDS-PAGE analysis was carried out to identify enzymes involved in the biodegradation, and a protein of size 56 kDa molecular weight was identified and confirming the presence of Pyrethroid hydrolase. The fragmented ß Cypermethrin metabolite was reconfirmed by GC–MS analysis, which confirmed the degradation of ß Cypermethrin. The peaks of metabolites were recognized as Benzylamine and related components, these are derivatives of ß cypermethrin in degraded by B. cereus. Hence, the study concludes that, the 56 kDa molecular weight Pyrethroid hydrolase synthesized by B. cereus has the efficiency to metabolize the ß Cypermethrin pesticide. Thus, the B. cereus could be most suitable agent for chemical pesticide degradation.