Enzyme and coenzyme levels, oxygen uptake and lactate production in synchronized cultures of Chinese hamster cells

Abstract

Summary The activities of five enzymes involved in glucose utilization were measured in synchronized cultures of Chinese hamster cells. In the 12 h after mitosis, the activity per cell of glucose-6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase, hexokinase, phosphoglucomutase, and glucose phosphate isomerase increased continuously to 180% of the initial values. The levels of oxidized and reduced pyridine nucleotides increased linearly, the ratio of oxidized-to-reduced forms remained constant, and the rates of oxygen consumption and lactate production were unchanged. These results suggest a stable basal cellular metabolism in these cells throughout interphase.