Abstract
Although circumvention of the problem of systemic toxicity during regional infusions was shown to be possible with S-182, an alkylating agent with a very short half-life, practical use of this drug was hampered by its lack of water solubility and damage to normal tissue near the injection site. Studies were undertaken to find an agent without such shortcomings and ethylene N-2-chloroethyl-N-methylglycine, diester (S-73), a stable water-soluble, bifunctional alkylating agent with esterase-susceptible bonds was studied further. This compound should be rapidly detoxified by esterases in blood and epithelial tissue. In dogs, low doses of S-73 (0.27 μm./kg.) injected into a branch of the mesenteric artery, about 10 cm. from the intestine, damaged the intestinal epithelium but no damage occurred when injected 20 cm. away. HN2 (0.06 μm./kg.) unlike S-73, did not lose its cytotoxic effect when injected twice as far from its target. High doses of S-192 (a monofunctional mustard which could result from the cleavage of S-73 by esterase) were innocuous when injected 10 cm. from the intestine. Bone marrow depression occurred only with high doses of S-73 (17.6 μm./kg.) injected into the left ventricle but not through the femoral vein. The significance of this observation is enhanced by the observation that 2.6 μm. per kilogram of HN2 by either route depresses bone marrow. In vitro studies, however, show that S-73 is not rapidly detoxified in blood, and suggest that much of the compound may be in the uncyclized form which is incapable of alkylating at the time it reaches the target. Nevertheless, in vivo studies reveal that considerable alkylation has occurred at the target. Furthermore, rapid detoxification of S-73 may be inferred by the higher doses required to effect regional damage, the relationship of its cytotoxic effect to distance from the target, and the lack of local effect of its presumed metabolite. Since very high doses of S-192 caused damage in the lungs of dogs, it is not surprising that equivalently high doses of S-73 are also damaging to lung. Preliminary clinical trials [2] in advanced cancer patients have shown that high doses of S-73 have not produced damage to either bone marrow or lung. Mucosal damage on intramesenteric arterial injection and spinal cord damage via the lower aorta in dogs as well as preliminary clinical observations have shown that S-73 is a potent cytotoxic agent when injected near a limited target. On the other hand high doses in a larger tumor-bearing area produced minimal damage to local tissue with no significant systemic effects. Whether clinical cancer can be specifically and significantly damaged with this new agent is in the process of investigation.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.