Abstract
AbstractThe activities of nine different enzymes (glucose‐6‐phosphatase, combined activities of glucose‐6‐phosphate dehydrogenase and 6‐phosphogluconate dehydrogenase, phosphohexose isomerase, aldolase, iso‐citric dehydrogenase, lactic dehydrogenase, glutamic‐oxalacetic transaminase, glutamic‐pyruvic transaminase and glutamic acid dehydrogenase) were measured in liver, skeletal muscle, cerebral cortex, hypothalamus, adrenal cortex and thyroid gland of hypophysectomized rats and rabbits. The water and fat contents were determined in liver and muscle. DNA and protein were measured in all tissues. After hypophysectomy the activities of the Embden‐Meyerhof enzymes were unaffected when the values were expressed per unit wet weight or per unit protein. When calculated on unit DNA the activities of almost all enzymes were in most tissues significantly decreased after hypophysectomy. This finding is due to the fact that except for cerebral cortex and hypothalamus in rabbits the DNA content of all tissues studied is significantly increased after hypophysectomy. The observed changes in enzyme activities and DNA content in the tissues are discussed in relation to hormonal and dietary influences on enzyme activities.
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