Abstract
Apical membrane vesicles were isolated from the confluent LLC-PK 1 cells by nitrogen cavitation and Mg/EGTA precipitation mfethods. The specific activities of marker enzymes for apical membranes were enriched 8- to 18-fold relative to those in the homogenate. d-[ 3H]Glucose uptake into the vesicles was stimulated in the presence of Na + gradient (overshoot phenomenon), and the values of apparent K m and V max for Na +-dependent component of d-glucose uptake were 0.3 mM and 5.8 nmol/mg protein per min, respectively.
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Schedule a callMore From: Biochimica et Biophysica Acta (BBA) - Biomembranes
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