Enzyme activities and glyphosate biodegradation in a riparian soil affected by simulated saltwater incursion

Changming Yang,Mengmeng Wang

doi:10.1038/npre.2011.6187.1

Abstract

AbstractSoil salinization due to saltwater incursion, is a major threat to biochemical activities and thus strongly alters biogeochemical processes in a freshwater riparian of coastal estuary region. A pot incubation experiment was conducted to investigate the effects of simulated saltwater incursion on some key enzymatic activities and biodegradation dynamics of herbicide glyphosate in a riparian soil in Chongming Island located in the Yangtze River estuary, China. The results showed that saltwater addition with 10% artificial seawater significantly increased the biodegradation efficiency of glyphosate with the lowest residual concentration among all the treatments. However, glyphosate degradation was markedly decreased in the riparian soil with high levels of saltwater treatment. As compared with no saltwater treatment, the half-lives for 20% and 50% seawater treatments were prolonged by 4.9% and 21.1%, respectively. Throughout the incubation period, saltwater addition with 10% seawater stimulated the enzymatic activities in the glyphosate-spiked riparian soil, as compared to the treatment with 0% seawater. Flourescein diacetate (FDA) hydrolysis rate, dehydrogenase activity (DHA), catalase activity, and alkaline phosphatase activity in the glyphosate-spiked riparian soil treated with 10% seawater were 68.5%, 49.2%, 38.7%, and 28.6% higher than those for no saltwater treatment, respectively. The effect of 20% seawater treatment on the glyphosate-spiked riparian soil enzymatic activities fluctuated between promotion and inhibition depending on the type of enzymes. Soil enzymatic activities were severely depressed by increasing salinity level with 50% seawater treatment significantly inhibited, relative to no saltwater treatment. Especially, FDA hydrolysis rate and DHA were decreased by 73.8% and 64.8%, respectively, as compared to no saltwater treatment. Glyphosate degradation percentages were strongly positively correlated to the FDA hydrolysis rate and DHA, indicating that as compared to the other enzymes, the two enzymes contributed more to the herbicide biodegradation in the salt-affected riparian soil.

Highlights

As an interface and transition zone between terrestrial and aquatic ecosystems, riparian wetlands and associated microbial, plant and fauna processes have multiple ecological and environmental functions such as riverside protection, water purification and herbicide nonpoint pollution control
A pot incubation experiment was conducted to investigate the effects of simulated saltwater incursion on some key enzymatic activities and biodegradation dynamics of herbicide glyphosate in a riparian soil in Chongming Island located in the Yangtze River estuary, China
(121.26° E, 31.55° N) in the Southwest of Chongming Island, where soil salinity is relatively slight due to receieving less seawater incursion. 200g dry weight equivalent BSMcontaminated riparian soil was weighed into a 500ml flask and statically incubated at 30

Summary

Introduction

As an interface and transition zone between terrestrial and aquatic ecosystems, riparian wetlands and associated microbial, plant and fauna processes have multiple ecological and environmental functions such as riverside protection, water purification and herbicide nonpoint pollution control. Soil salinization due to saltwater incursion, is a major threat to biochemical activities and strongly alters biogeochemical processes in a freshwater riparian of coastal estuary region. A pot incubation experiment was conducted to investigate the effects of simulated saltwater incursion on some key enzymatic activities and biodegradation dynamics of herbicide glyphosate in a riparian soil in Chongming Island located in the Yangtze River estuary, China

Materials and Method

Findings

FDA hydrolysis rate