Enzymatically Crosslinked In Situ Synthesized Silk/Gelatin/Calcium Phosphate Hydrogels for Drug Delivery.

Andra Grava,Karina Egle,Arita Dubnika

doi:10.3390/ma14237191

Abstract

Our research focuses on combining the valuable properties of silk fibroin (SF) and calcium phosphate (CaP). SF is a natural protein with an easily modifiable structure; CaP is a mineral found in the human body. Most of the new age biocomposites lack interaction between organic/inorganic phase, thus SF/CaP composite could not only mimic the natural bone, but could also be used to make drug delivery systems as well, which can ensure both healing and regeneration. CaP was synthesized in situ in SF at different pH values, and then crosslinked with gelatin (G), horseradish peroxide (HRP), and hydrogen peroxide (H2O2). In addition, dexamethasone phosphate (DEX) was incorporated in the hydrogel and drug delivery kinetics was studied. Hydrogel made at pH 10.0 was found to have the highest gel fraction 110.24%, swelling degree 956.32%, and sustained drug delivery for 72 h. The highest cell viability was observed for the hydrogel, which contained brushite (pH 6)—512.43%.

Highlights

Bone and cartilage damage is the main cause of osteoarthritis, but other factors, such as joint injures, obesity, bone deformities, multiple stress on joints, and metabolic diseases, increase it [1]
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calcium phosphates (CaP) can be incorporated in Silk fibroin (SF) by in situ synthesis, which provides organic and inorganic phase interaction

Summary

Introduction

Bone and cartilage damage is the main cause of osteoarthritis, but other factors, such as joint injures, obesity, bone deformities, multiple stress on joints, and metabolic diseases, increase it [1]. Osteoarthritis is the eighth leading health condition associated with disability [2]. Muscle mass tends to decrease with age, which affects musculoskeletal functions, for example, lower gait speed and weaker hand grip [2]. Defective areas can be mended and cured. The new-age materials are no longer one-phase materials as they cannot provide all the necessary properties. This is where composite materials come in

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