Abstract
In this paper, we proposed an enzymatic urea sensor using urease-magnetic beads (MBs) modified graphene oxide (GO)/titanium dioxide (TiO <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sub> ) composite layers. The urea sensor was prepared by screen-printing technology and radio frequency sputtering. TiO <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sub> sensing film was deposited onto polyethylene terephthalate substrate, which serves as an efficient matrix for immobilization of urease. Preparation of GO/TiO <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sub> films was completed after GO being deposited onto the TiO <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sub> film. Moreover, the characterization and sensing properties of the urea sensor were performed by energy dispersive spectrometer, scanning electron microscope, atomic force microscopy, electrochemical impedance spectroscopy, and voltage-time measurement system. The results indicated that the GO/TiO <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sub> urea sensor enhances its performance of average sensitivity, response time, and drift rate after modifying the sensing membrane though MBs.
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