Abstract
To enzymatically transform protopanaxatriol by using β-glucosidase from Thermotoga neapolitana (T. neapolitana) DSM 4359. Recombinant β-glucosidase was purified, which molecular weight was about 79.5kDa. High levels of ginsenoside were obtained using the follow reaction conditions: 2mgml-1 ginsenoside, 25 U ml-1 enzyme, 85°C, and pH 5.0. β-glucosidase converted ginsenoside Re to Rg2, Rf and Rg1 to APPT completely after 3h under the given conditions, respectively. The enzyme created 1.66mgml-1 Rg2 from Re with 553mgl-1 h-1, 0.85mgml-1, and 1.01mgml-1 APPT from Rg1 and Rf with 283 and 316mgl-1 h-1 APPT. β-glucosidase could be useful for the high-yield, rapid, and low-cost preparation of ginsenoside Rg2 from Re, and APPT from the ginsenosides Rg1 and Rf.
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