Enzymatic synthesis, structure of isomalto/malto-polysaccharides from linear dextrins prepared by retrogradation

Abstract

Isomalto/malto-polysaccharides (IMMPs) with degree of polymerization (DP) 10–100 have novel potential applications, including enhanced solubility and anti-inflammatory. However, there are minimal synthetic methods for preparing IMMPs with a relatively higher DP, which is due to the lack of suitable molecular weight linear dextrins (I-LDs). The existing I-LDs preparation methods have disadvantages, such as low yield and uncontrollable molecular weight. Therefore, this study proposes a method for preparing soluble linear dextrins (S-LDs, Mw = 2.1 kDa) by low-temperature retrogradation from debranched waxy corn starch (Mw = 3.0 kDa). S-LDs reacted with 4,6-α-glucanotransferase GtfB-ΔN from Limosilactobacillus reuteri 121 to yield IMMPs with 12.3 kDa Mw and 83.8% α1 → 6 linkages content. Process monitoring revealed the synthesis mechanism and a detailed reaction process. Finally, IMMPs were identified by enzyme fingerprinting as α1 → 6 chains with α1 → 4 fragments inlaid at the reducing, non-reducing end, and middle part. This study provides a new synthesis method and more structural information for IMMPs.