Enzymatic synthesis of sugar fatty acid esters in organic solvent and in supercritical carbon dioxide and their antimicrobial activity

Abstract

Lipase-catalyzed synthesis of different sugar fatty acid esters was performed in high yields in 2-methyl-2-butanol at atmospheric pressure and in supercritical carbon dioxide (SC CO 2) at 10 MPa. Influence of molecular sieves concentration on conversion in SC CO 2 was studied. Growth inhibitory effect of commercial sucrose fatty acid esters and enzymatically synthesized sucrose and fructose fatty acid esters on Gram-positive and Gram-negative micro-organisms, as well as on yeast was tested. Sucrose laurate inhibited the growth of Bacillus cereus food poisoning bacteria at a concentration of 9.375 mg/ml.