Enzymatic synthesis of structured lipids with behenic acid at the sn-1, 3 positions of triacylglycerols.

Abstract

A long chain saturated fatty acid (SFA), behenic acid, is incorporated into the sn-1, 3 positions of triacylglycerols in palm olein (POo) and high-oleic sunflower oil (HOS) by solvent-free interesterification catalyzed by Lipozyme RM IM. The enzymatic interesterified HOS (EIE-HOS) yielded 76.5% of BOO and BOB as compared to 45.6% in POo (EIE-POo). The sn-2 position of EIE-HOS displayed 5.3mol% of SFA which is significantly lower compared to 13.5mol% in EIE-POo (P<0.001). The sn-1, 3 positions of EIE-POo exhibited greater amount of behenic acid (82.0mol%) in relation to EIE-HOS (64.0mol%) (P<0.001). Due to the greater variety of constitutive triacylglycerol, EIE-POo showed greater differences between onset (To) and offset temperature (Tf) in the melting endotherms (76.99°C) as compared to EIE-HOS (68.65°C), and may offer more intensive cooling sensation and flavor release.