Abstract
AbstractEnzymatic synthesis of para‐lacto‐N‐hexaose and its isomeric structures as well as those α1,2‐fucosylated variants naturally occurring in human milk oligosaccharide (HMOs) was achieved using a sequential one‐pot enzymatic system. Three glycosylation routes comprising bacterial glycosyltransferases and corresponding sugar‐nucleotide‐generating enzymes were developed to facilitate efficient production of extended type‐1 and type‐2 N‐acetyllactosamine (LacNAc) backbones and hybrid chains. Further fucosylation efficiency of two α1,2‐fucosyltransferases on both type‐1 and type‐2 chains of the hexasaccharide was investigated to achieve practical synthesis of the fucosylated glycans. The availability of structurally defined HMOs offers a practical approach for investigating future biological applications.magnified image
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