Enzymatic Synthesis of Glutamine in Higher Plants

Abstract

volumes of chilled acetone in a Waring Blendor at Io C. The suspension was filtered with suction at Io C, and the residue sucked dry in a stream of cold air. The operation was repeated, and the coarse powder was sifted through a screen. The resulting powder was stored in a desiccator at Io C. Active extracts of acetone powders were prepared essentially in the manner described by Speck (10). Unless otherwise indicated, the complete reaction system contained: 0.05 M potassium phosphate buffer (pH 7.0), 0.05 M sodium L-glutamate,