Abstract
AbstractA method for the enzymatic synthesis of [1‐14C‐N‐acetyl, P18O2] cytidine monophosphate neuraminic acid (CMP‐NeuAc) is described. Central to the synthesis of [1‐14C‐N‐acetyl, P18O2]CMP‐NeuAc was the enzymatic preparation of [γ‐P18O3]ATP for use in a reaction with uridine kinase and cytidine to provide 5′‐[P18O3]CMP. The [1‐14C‐N‐acetyl, P18O2]CMP‐NeuAc isotopomer was then synthesized from a reaction involving nucleoside monophosphate kinase, pyruvate kinase and CMP‐NeuAc synthetase. The isolated reaction yield was 35%. Copyright © 2004 John Wiley & Sons, Ltd.
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