Abstract
Enzymatic synthesis and the reverse transcription of RNAs containing 2'-O-carbamoyl uridine were evaluated. A mild acidic deprotection procedure allowed the synthesis of 2'-O-carbamoyl uridine triphosphate (UcmTP). UcmTP was incorporated correctly into long RNAs, and its fidelity during reverse transcription using SuperScript III was sufficient for RNA aptamer selection.
Highlights
A mild acidic deprotection procedure allowed the synthesis of 20-O-carbamoyl uridine triphosphate (UcmTP)
UcmTP was incorporated correctly into long RNAs, and its fidelity during reverse transcription using SuperScript III was sufficient for RNA aptamer selection
Chemical synthesis can introduce a wide variety of chemical modifications into RNA aptamers that are selected using canonical nucleoside triphosphates (NTPs)
Summary
Enzymatic synthesis and reverse transcription of RNAs incorporating 20-O-carbamoyl uridine triphosphate† Enzymatic synthesis and the reverse transcription of RNAs containing 20-O-carbamoyl uridine were evaluated. UcmTP was incorporated correctly into long RNAs, and its fidelity during reverse transcription using SuperScript III was sufficient for RNA aptamer selection.
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