Abstract
Current zonal electrophoretic techniques for the separation of alkaline phosphatase isoenzymes are unsatisfactory for “in depth” clinical use. A recently described isoelectric focusing technique using agarose gel containing both carrier ampholytes and separators over a pI range of 3.01 to 4.86, suggests at least twelve bands of ALP activity. Four of the most acidic bands appear in serum, with activity above reference range, in the presence of clinically confirmed hepatic disease. It is thought that each band represents ALP secreted by a distinct hepatic cellular architecture. Various hepatic diseases are illustrated by patient profiles, from which a pattern recognition developed. It is emphasized that the patterns are reflections of serum ALP activity, without pre-treatment.
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