Abstract
AbstractAn enzyme reaction system with coenzyme regeneration was investigated for L‐alanine production. Alanine dehyrogenase L‐alanine: NAD+ oxidoreductase (EC 1.4.1.1)] from Corynebacterium flaccumfaciens AHU‐1622 was used as the catalyst for reductive amination of pyruvate to L‐alanine. NAD‐ and NADP‐linked malic enzyme [L‐malate: NAD(P)+ oxidoreductase (EC 1.1.1.39)] from Pseudomonas diminuta IFO‐13182 was used for the regeneration of NADH. Optimum conditions for L‐alanine production were determined, including L‐malic acid concentration, MgCl2 concentration and pH. Under suitable conditions, the conversion of L‐malic acid to L‐alanine reached 95% after 72 h of incubation at 30°C, yielding 106 mol/m3 of L‐alanine. The L‐alanine produced was purified in crystal form; its purity was 99.4%, based on HPLC analysis.
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