Abstract
The intermediary metabolism of active denitrifying populations cultivated on glucose and on malate, was investigated. Each carbon source induced a specific metabolic pattern. Glycolytic enzymes were more active in the glucose culture than in the malate culture (6:1), as were the pentose phosphate shunt enzymes (32:1), while the citric acid cycle enzymes were more active in the malate culture (7:1). Cytochromes were more abundant in the malate than in the glucose culture. The malate culture possessed the ability to link the citric acid cycle with the process of reversed glycolysis.
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