Enzymatic modification of triolein: Incorporation of caproic and butyric acids to produce reduced‐calorie structured lipids

Abstract

AbstractLipase‐catalyzed acidolysis of triolein with caproic and butyric acids was performed to produce reduced‐calorie structured lipids (SL). The SL were obtained by incubating a 1:4:4 mole ratio of triolein, caproic acid, and butyric acid, respectively, with 10% of lipase (w/w of total substrates) in 1.5 mL hexane at 55°C for 24 h. Of nine commercially avaialble lipases screened, IM60, which contains the lipase from Rhizomucor miehei, was the most effective and produced 13 mol% unreacted triolein, 49% disubstituted, and 38% monosubstituted triacylglycerols that contained short‐chain fatty acids. The products were analyzed by reverse‐phase high performance liquid chromatography with an evaporative light‐scattering detector. Reaction parameters studied included time course, temperature, enzyme load, and substrate mole ratio. The yields obtained demonstrate that a structured lipid with long‐chain and short‐chain fatty acids can be synthesized by using IM60 lipase in organic medium.