Abstract
When alginate is split by an alginate lyase, an unsaturated unit with a strong absorbance at 230 nm is formed at the new non-reducing end. At excessive enzyme levels, the absorbance will approach an apparent endpoint level that reflects the initial substrate concentration. On this basis, a simple and rapid assay has been developed to detect and quantify alginates in solution in the 0·01–1 mg/ml concentration range. A combination of purified guluronate lyase from Klebsiella pneumoniae and purified mannuronate lyase from Haliotis tuberculata is applied to eliminate the influence of the specific composition of the alginates. Applied separately and in combination, these enzymes may also give information about the alginate structure in accordance with their block distribution structure determined by NMR.
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