Abstract
A sensitive method for the estimation of residual organophosphorus compounds in water after treatment with chlorine-containing oxidants has been developed. The method is based on the inhibiting action of organophosphorus compounds towards the enzyme-catalyzed hydrolysis of esters. After the oxidants have been quantitatively destroyed by a mixture of NaHCO3 and NaNO2 small amounts of O-ethyl S-(N, N-diisopropylamino)ethyl methylphosphonothioate (MESP), dichlorvos (DDVP) and dimethoate are determined by using horse-serum cholinesterase with 2,6-dichloroindophenyl acetate as the substrate. A linear response range of 2 of 3 orders of magnitude and recovery within 100 ± 5% has been generally obtained.
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