Abstract
Reliable assay systems were developed for the detection and quantitation of butanol dehydrogenase and butyraldehyde dehydrogenase in extracts of Clostridium acetobutylicum. Butanol dehydrogenase was NADPH-dependent. The enzyme could be sparated by ultracentrifugation from a NADH-specific enzyme which probably represents the ethanol dehydrogenase but which also reacted with butyraldehyde to form butanol. Butyraldehyde dehydrogenase proved to be NADH-specific. All enzymes were induced shortly before butanol formation began. Specific activities decreased at the end of the fermentation process. An explanation for contradictory data in the literature is proposed.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
