Enzymatic hydrolysis of steam-exploded sugarcane bagasse by adding natural Sapindus peel

Abstract

KEYWORD: sugarcane bagasse; steam-explosion; enzymatic hydrolysis; Sapindus peel ABSTRACT: Steam explosion pretreatment was a feasible method to treat sugarcane bagasse. The results showed that the hemicelluloses can be effectively removed during the pretreatment and in- crease the ability of enzymatic hydrolysis. The highest glucose yield was achieved at 74.99%, which was increased by 57.86% with the same enzyme loadings 30 FPU/g-cellulose compared with the un- treated sugarcane bagasse. The glucose yield of steam-exploded sugarcane bagasse can be signifi- cantly improved from 36.46% to 74.99% with the increment of cellulase. Sapindus peel contained natural surfactant can evidently reduce the surface tension of hydrolysis broth and improve the abili- ty of enzymatic hydrolysis at an economic concentration (1.2 g/L). The highest glucose yield was of 80.99% with cellulase loading of 10 FPU/g-cellulose and addition of 1.2 g/L Sapindus peel, which suggest that 10 FPU/g-cellulose was suitable for lignocellulose saccharification industry.