Abstract
A preliminary study was performed on enzymatic hydrolysis process for treating empty fruit bunch (EFB) fibre. The bioconversion of cellulose hydrolysis was carried out with soluble cellulase from Trichodermareesei as the biocatalyst. Crucial trends such as substrate and enzyme loading influencing the enzymatic reaction were also studied in order to enhance the cellulose conversion. The results indicate that as the enzyme loading was increased, the EFB conversion also increased until it reached 115.63 FPU/g of enzyme concentration, beyond this values, the reverse occurred. On the other hand, as the substrate loading was increased the conversion decreased. Inhibition of enzyme adsorption by hydrolysis products appear to be the main cause of the decreasing conversion at increasing enzyme loading and substrate loading.
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