Abstract
Sixty different strains of Streptomyces were tested for their ability to release reducing sugars form wheat straw. The most active three strains (Streptomyces mutabilis, S. chromofuscus, and S. cyanoviridis) were chosen for further studies. The action of the two enzymes laminarinase and cellulase on eight different substrates (starch, carboxymethylcellulose, wheat straw, waste paper, cellulose, mannose, glucose and glycerol) was measured. There was a decrease in the activity of laminarinase, by using different carbon sources in the following order: carboxymethylcellulose, mannose, wheat straw, waste paper, cellulose, glucose, starch and glycerol at the optimum pH 7 and temperature of 50°C. The sequence of cellulase-substrate affinity was: wheat straw waste paper, cellulose, mannose, glucose, starch, glycerol, and carboxymethylcellulose at the optimum pH 6 and temperature of 50°C. Thin layer chromatography was performed to determine the different released sugars. Hexose and pentose spots were detected.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
