Enzymatic Hydrolysis of Alginate to Produce Oligosaccharides by a New Purified Endo-Type Alginate Lyase.

Benwei Zhu,Limin Ning,Meijuan Chen,Heng Yin,Yuguang Du

doi:10.3390/md14060108

Abstract

Enzymatic hydrolysis of sodium alginate to produce alginate oligosaccharides has drawn increasing attention due to its advantages of containing a wild reaction condition, excellent gel properties and specific products easy for purification. However, the efficient commercial enzyme tools are rarely available. A new alginate lyase with high activity (24,038 U/mg) has been purified from a newly isolated marine strain, Cellulophaga sp. NJ-1. The enzyme was most active at 50 °C and pH 8.0 and maintained stability at a broad pH range (6.0–10.0) and temperature below 40 °C. It had broad substrate specificity toward sodium alginate, heteropolymeric MG blocks (polyMG), homopolymeric M blocks (polyM) and homopolymeric G blocks (polyG), and possessed higher affinity toward polyG (15.63 mM) as well as polyMG (23.90 mM) than polyM (53.61 mM) and sodium alginate (27.21 mM). The TLC and MS spectroscopy analysis of degradation products suggested that it completely hydrolyzed sodium alginate into oligosaccharides of low degrees of polymerization (DPs). The excellent properties would make it a promising tool for full use of sodium alginate to produce oligosaccharides.

Highlights

Alginate is a linear acidic polysaccharide consisting of 1,4-linked β-D-mannuronate (M) and its C5 epimer α-L-guluronate (G) [1]
The hydrolysates of alginate, have attracted increasing attention due to their obvious activities, excellent gel properties and good solubility, which is essential for absorption
A new alginate lyase with high activity has been purified from Cellulophaga sp

Summary

Introduction

Alginate is a linear acidic polysaccharide consisting of 1,4-linked β-D-mannuronate (M) and its C5 epimer α-L-guluronate (G) [1]. Alginate is an important source for food and medicine It possesses various kinds of physiological activities, such as anti-tumor [3,4], immune-regulating [5,6] and growth-promoting [7]. The hydrolysates of alginate, have attracted increasing attention due to their obvious activities, excellent gel properties and good solubility, which is essential for absorption They can be used as growth promoters for plants and therapeutic agents such as anticoagulants and tumor inhibitors [10].

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