Abstract

A current hot topic in the field of environmental protection and creation of valuable new products is represented by the recovery of food waste from the meat sector. The present study proposed the recovery of bovine hides by means of a sustainable method. Enzymatic hydrolysis was selected as a potential greener methodology for use in the production of protein hydrolysates to be applied on an industrial scale. For this purpose, the enzymatic hydrolysis of bovine hides with Alcalase was investigated following a multiscale approach: lab scale, medium scale and semi-industrial pilot plant. Alcalase turned out to be highly efficient due to its ability to degrade collagen, the main protein of hides. Under the optimized conditions, hides hydrolysis at lab and medium scales enabled to obtain about 85% of protein solubilisation after 6 hours, with a consistent release of free amino acids and a degree of hydrolysis of 17-19%. However, in the pilot plant, the solubilisation decreased due to difficulties in bovine hides mixing in the reactor, which was compensated with a longer reaction time to reach almost a complete protein solubilisation (∼98%). Therefore, the present data demonstrates the applicability of the process at semi-industrial scale for protein recovery with reduced amount of waste by-products.

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