Abstract
A β-glueoside of D-pantothenic acid was formed from D-pantothenic acid and β-glucosyl donors such as cellobiose, phenyl-β-D-glucoside, salicin, and 4-methylumbelliferyl-β-D-glueoside and naphthol AS-BI-β-D-glucoside by various β-glucosidases, i.e., almond β-glucosidase, cellulase type II and III, naringinase, and hesperiginase. The compound was isolated from a reaction mixture of almond β-glucosidase by treatment with active charcoal, Amberlite CG-50, and DEAE-cellulose column chromatography, paper chromatography, and Sephadex G-10 gel filtration. Then, the compound was characterized as 4'-O-(β-D-glucopyranosyl)-D-pantothenic acid by various analytical methods including bioassay, paper chromatography, NMR and specific optical rotation. The microbiological activities of the compound were also determined.
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