Abstract
Penthorum chinense Pursh (PCP) is a kind of functional food or medicine for liver protection. In the present work, Plackett-Burman design, steepest ascent method, and response surface methodology (RSM) were employed to obtain maximum total sugar yield. The experimental yield of 6.91% indicated a close agreement with the predicted yield of 7.00% of the model under optimized conditions. The major polysaccharide fraction (PCPP-1a) from PCPP was purified and identified as acidic polysaccharides with a high content of uronic acid (FT-IR, UV, HPGPC). PCPP had similar monosaccharide profile with PCPP-1a but was rich in galacturonic acid (HPLC). Both of PCPP and PCPP-1a possessed strong hydroxyl radical scavenging, DPPH radical scavenging, and Fe2+ chelating activities. Moreover, they were revealed to show strong anti-inflammatory activities by inhibiting NO, TNF-α, and IL-1β release compared to LPS treatment in RAW264.7 cells. These data suggest that the polysaccharides from PCP could be potential natural products for treating ROS and inflammatory-related diseases.
Highlights
Polysaccharide, a crucial biomacromolecule in living organisms, is one of the most abundant storage carbohydrates of plants [1]
The results showed that the monosaccharide compositions of Penthorum chinense Pursh polysaccharides (PCPP) were more complicated than that of PCPP (6.91%). The main fraction (PCPP-1a) and implied that crude PCPP was the typically acidic polysaccharide rich in galacturonic acid
The results indicated that PCPP and PCPP-1a at the tested concentrations of 10, 25, 50, 100, and 100 μg.mL−1 (Figure 6(a)) showed insignificant influence on RAW264.7 cells viability (p>0.05)
Summary
Polysaccharide, a crucial biomacromolecule in living organisms, is one of the most abundant storage carbohydrates of plants [1]. Botanical polysaccharides have attracted increasing attention for their health-related benefits and therapeutic use with relatively high security [6, 7] They have immense potential industrial applications in pharmaceuticals [8], cosmetics [9], foods [10], and materials [11]. Penthorum chinense Pursh (PCP, Saxifragaceae) mostly distributed in China (Miao ethnomedicine), eastern Russia, Japan, and Korea [12,13,14,15,16]. It has been used traditionally as functional food or traditional Chinese medicine for treating liver disease [17, 18]. Studies on the extraction, characterization, and bioactivities of Penthorum chinense Pursh polysaccharides (PCPP) are limited
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