Abstract
The aim of this study was to optimise pectin extraction from artichoke by-products with Celluclast®1.5L using an experimental design analysed by response-surface methodology (RSM). The variables optimised were artichoke by-product powder concentration (2–7%, X1), enzyme dose (2.2–13.3 U g−1, X2) and extraction time (6–24 h, X3). The variables studied were galacturonic acid (GalA) (R2 93.9) and pectic neutral sugars (R2 92.8) content and pectin yield (R2 88.6). In the optimum extraction conditions (X1 = 6.5%; X2 = 10.1 U g−1; X3 = 27.2 h), pectin yield was 176 mgg−1 dry matter (DM). Considering 27.2 h of treatment as the +α value given by the design, the extraction time was increased up to 48 h obtaining a yield of 221 mg g−1 DM. The enzymatic method optimised allows obtaining artichoke pectin with good yield, high GalA (720 mg g−1 DM) and arabinose (127.6mgg−1 DM) contents and degree of methylation of 19.5%.
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