Abstract
Abstract Anomeric configuration of oligosaccharides usually is established by specific glycosidases. For this purpose detergents achieving water solubility of primarily insoluble glycosphingolipids as substrates have been replaced by delipidated hum an serum high density lipoproteins. The new method, tested by several well characterized glycosphingolipids and glycosidases, finally was applied to the evaluation of anomeric structures of two blood-group B active glycosphingolipids [ceramide hexa-saccharide (B-I) and ceramide octasaccharide (B -II)] from hum an erythrocyte membranes. In both B-I and B-II, α-glycosidic linkage was dem onstrated for the term inal galactose and fucose residues. β-glycosidic linkage has been evaluated for backbone saccharides. Together with the results pre viously obtained by composition analysis, linkage analysis and sequence analysis the following complete structure can be established: B -I: Galα1 → 3Gal (2 ← 1αFuc)β1 → 4GlcNAcβ1 → 3Galβ1 → 4Glcβ1 → 1Cer; B-II: Galα1 → 3Gal (2 ← 1αFuc)β1 → 4GlcNAcβ1 → 3Galβ1 → 4GlcNAcβ1 → 3Galβ1 → 4Glcβ1 → 1Cer.
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