Abstract
The detection of carbamate pesticides by an enzyme-inhibition method on impregnated (ethylene glycol, triethylene glycol, beta,beta'-oxydipropionitrile) cellulose thin layer plates is described. The enzyme-substrate combination: pig liver esterase-indophenylacetate (chromogenic substrate) was the most sensitive. The following parameters concerning the sensitivity of the method were investigated: enzyme source, substrate, pH, inhibition time, inhibition temperature, thickness of the cellulose layer, stationary phase.
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