Abstract
The deiodination of L-DIT in vitro by thyroid and liver tissue was studied. Supplements of FAD stimulated deiodination in dilute thyroid homogenates and subcellular particulate preparations enriched with NADPH. Sodium dithionite (0.05M) stimulated deiodination in the absence of NADPH and its effect was also enhanced by FAD. The products of deiodination were the same in NADPH and dithionite-supplemented systems in the presence of FAD. The stimulating effect of FAD under these conditions was detectable at micromolar concentration, was not dependent upon light or oxygen, and appeared to be enzymatically mediated. The distributions of deiodinating activity supported by NADPH and by dithionite among thyroid subcellular fractions were similar, both being predominantly microsomal; NADPHdependent activity appeared more sensitive to heat and aging. The stimulating effect of dithionitedithionite could not be explained on the basis of reduction of endogenous NADP with formation of effective concentration of NADPH si...
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
