Abstract
Pullulan was modified using three different methods: a) chloroformate activation and subsequent reaction with 2-hydroxypropylamine, b) succinoylation and c) partial periodate oxidation and subsequent reduction of the aldehyde groups. Degradation of pullulan and pullulan derivatives by α-amylase, β-amylase and lysosomal enzymes was investigated. It was observed that the rate of degradation decreased with increasing degree of chemical modification of the parent polysaccharide. The stability of pullulan and dextran was tested in serum and liver homogenate.
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