Abstract

Using paper chromatographic methods the glycosidic constituents of freshly gathered leaves of Digitalis lanata have been examined before and after exposure to a limited period of enzymatic action. It appears that the original plant does not contain desglucoglycosides in quantities detectable by very sensitive methods, but that after exposure to enzyme degradation under defined conditions, glucose only is removed from the lanatosides to give acetyl digoxin, acetyl digitoxin, and probably acetyl gitoxin. The separation and identification of the less water soluble digitalis glycosides, some of their acetyl derivatives, and their genins, has been helped by the use of a new temperature-insensitive method of paper chromatography.

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