Abstract
Enzymatic conversion of oligomeric xylose and insoluble xylan remaining after effective pretreatment offers significant potential to improve xylan-to-xylose yields while minimizing yields of degredation products and fermentation inhibitors. In this work, a commercial enzyme cocktail is demonstrated to convert up to 70 % of xylo-oligomers found in dilute acid-pretreated hydrolyzate liquor at varying levels of dilution when supplemented with accessory enzymes targeting common side chains. Commercial enzyme cocktails are also shown to convert roughly 80 % of insoluble xylan remaining after effective high-solids, dilute acid pretreatment.
Highlights
The goal of reducing cellulosic ethanol process cost drives the continued pursuit of higher conversion of xylan to xylose
Enzyme catalyzed hydrolysis of xylooligosaccharides and insoluble xylan remaining after dilute-acid pretreatment offers significant potential to increase total xylose yield without further degradation product formation
Lower severity pretreatments focused on the minimization of product degradation have resulted in increased proportions of xylan remaining as xylo-oligomers and insoluble xylan after pretreatment, inspiring the pursuit of a variety of supplemental methods of xylan conversion
Summary
The goal of reducing cellulosic ethanol process cost drives the continued pursuit of higher conversion of xylan to xylose. Enzyme catalyzed hydrolysis of xylooligosaccharides and insoluble xylan remaining after dilute-acid pretreatment offers significant potential to increase total xylose yield without further degradation product formation. Lower severity pretreatments focused on the minimization of product degradation have resulted in increased proportions of xylan remaining as xylo-oligomers and insoluble xylan after pretreatment, inspiring the pursuit of a variety of supplemental methods of xylan conversion. Enzymatic conversion of post-pretreatment xylan and xylo-oligomers is promising because of the potential to produce significant amounts of monomeric xylose while reducing degradation product and inhibitor formation [1]. The current work demonstrates the effectiveness of enzymes in converting xylooligomers and insoluble xylan to xylose using pilot-scale dilute acid-pretreated corn stover. A commercial enzyme cocktail was used to demonstrate the conversion of insoluble xylan remaining after pretreatment to monomeric xylose. Addition of these enzymes only increased the overall enzyme usage by 6 % over cellulase loaded at 20 mg protein per gram cellulose
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
