Abstract
A PREVIOUS report from this laboratory1 gave evidence that histidine is converted to glutamic acid in vivo and in vitro. The in vitro experiment, done with guinea pig-liver slices, is subject to the criticism that degradation of α-formamidinoglutaric acid, the principal conversion product, may have occurred non-enzymatically. Direct evidence for such a conversion has been found so far only in micro-organisms adapted to histidine2. I have now shown the presence in rat liver of an enzyme which degrades α-formamidinoglutaric acid (N-formiminoglutamic acid) to glutamic acid.
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