Abstract
This chapter describes the enzymatic biodiesel production via transesterification of vegetable oils. Additionally, a new kind of enzymatic biodiesel is proposed where a.o. much less glycerol waste is produced and an up to 10% higher product yield is obtained. An overview is given of enzymes used as well as verification with experimental results. The literature review shows that there is general consensus in the studies that the enzymatic production of biodiesel is a superior method as compared to conventional chemical transesterification, considering the lower complexity of the reaction process and the absence of waste products, in particular glycerin and soap (produced due to presence of free fatty acids in the waste oil), which will create environmental problems if disposal is not handled appropriately. Reuse of the enzymes is essential for the economy of the process. A method for enzyme reuse is tested and described.
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