Enzymatic assays based on the coulometric microflow titration of ammonia and carbon dioxide

Abstract

Rapidly and precisely working microflow titrations are suitable to follow enzyme catalyzed reactions producing ammonia and carbon dioxide. Ammonia and carbon dioxide were separated from the reaction zone by a gas dialysis step. Highly precise and accurate determinations of urea, glutamine, glutamate, asparagine, carbon dioxide and ammonia can be implemented. The enzymes are immobilized covalently on a preactivated membrane sandwiched with a microporous Teflon membrane. A compact and fully automated microcoulometric measuring system for fast and long term stable enzymatic determinations was developed.