Abstract
In the twenty-first century, anaerobic digestion (AD) is considered to be the most efficient method among various other classical municipal solid waste management (MSWM) practices, which stabilize the generated organic fraction of municipal solid waste (OFMSW). Hydrolysis in AD is where the insoluble polymers in the procured OFMSW are converted into simpler soluble monomers by the fermentative bacteria, which generate certain extracellular enzymes known as hydrolases. These enzymes catalyze all the reactions. The hydrolysis being the rate-limiting step thus plays one of the crucial roles in the anaerobic decomposition of OFMSW by converting it into stable by-products. Chemically, hydrolysis can be defined as the breakdown of long-chain biomolecules into corresponding short chains in the presence of water. Biologically, hydrolysis acts through the influence of enzymes. The present research reviewed the potential, functional routes, and behavior of hydrolytic enzymes and studied the suitability of the enzyme cellulase in improving the performance of AD of OFMSW using a laboratory-scale setup.
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