Enzymatic and Toxicological Analysis of Vranaharin Ayurvedic Oil: An Experimental Study from a Clinical Perspective for Topical Applications on Necrotic Wounds

Abstract

Vranaharin, an ayurvedic oil manufactured by MMCA Remedies Pharmaceuticals, Karnataka, India, is a red-colored drug, prepared from several traditionally used plant ingredients and is used in postoperative dressings, bedsores, burns, cuts, wounds, diabetic ulcers, abscesses, skin infections, nail fungus, herpes and so on. This research aims to investigate Vranaharin oil, to understand its side-effects such as antioxidant enzymes alteration in the cerebral cortex of the brain and liver of developing chick embryos, pathological effect/antimicrobial nature, antioxidant ability and hemolytic activity. This study is designed to give an overall toxicological analysis of the selected drug. The oil which is used for topical applications of necrotic wounds is observed to cause angioneogenesis in necrotic wound bed within a short span of application. The oil has been investigated for the first time to assess the systemic effect of local application in terms of possible genotoxicity and embryonic toxicity. The study concludes that the oil can be effectively applied over the necrotized wound bed for a short period and its long-term application needs to be further investigated. Setting of maximum dose needs to be standardized as it caused notable fluctuations to enzyme equilibrium and variations in superoxide dismutase, catalase, glutathione peroxidase and glutathione- S-transferases levels when higher concentrations were used in chick embryos. Its topical application is highly recommended, especially in necrotizing wounds due to its excellent antioxidant, and antimicrobial potential. The angiogenic potential of the oil in avascular wounds is a very interesting phenomenon that is being investigated in our laboratory.