Enzymatic and feeding behaviour of Argopecten purpuratus under variation in salinity and food supply

Abstract

Argopecten purpuratus is a bivalve filter feeder inhabiting protected areas of the Peruvian coastline and northern and central Chile. The species is commercially valuable and the natural stocks are over-exploited in Chile, mainly in the central region. Expansion of aquaculture farms to the geographically protected coastline of southern Chile is a potential solution to this problem. So, the objective of the present study was to determine the acute feeding and digestive response of A. purpuratus in relation to short acclimation periods (1, 3 and 7days) and exposure to variable salinity (24‰, 27‰ and 30‰) with two different diets. The feeding behaviour of A. purpuratus follows that described for other species of bivalves whereby the decrease in salinity produces a reduction in the clearance and ingestion rates. This behaviour is not affected by the diet type or the 7 day acclimation period. Lower values of absorption efficiency are associated with increasing food concentration. The response of the AE at 24‰ salinity and low organic content diet indicates that the interaction of low salinity and low organic content diet reduces the AE to values approaching zero. The enzymatic investment of A. purpuratus at low salinity (24‰) is similar for both diets and more reduced than for 30‰ and 27‰ salinity. The results show that osmotic stress leads to a reduction of the enzymatic response capacity. The multiple regression analysis indicates that different responses are observed as a function of the enzymatic activity and the organ. A reduction of the carbohydrase activities in the digestive gland was observed to low salinity (24‰), specifically amylase and celullase. The results of this study are ecophysiologically relevant and highlight a relationship between digestive enzymatic activity and salinity, and may explain the possible differences in the energetic balance of A. purpuratus in response to changes in salinity.